| 1 | Rari Nantes Aprilia | Sbarrini Morgana Maria, Ciardo Gaia, Scaringella Flavia, Pizzo Eleonora | 02'26''70 | 262 |  |  | |
50m 37.20 (37.20) 100m 1:14.50 (37.30) [1:14.50] 150m 1:48.10 (33.60) 200m 2:26.70 (38.60) [1:12.20] |
| 2 | Kristall Sporting Club SS | Oniarti Elisa, Ichim Vanessa Ionela, Palmucci Valeria, Esposito Sara | 02'33''70 | 228 | | | |
50m 41.40 (41.40) 100m 1:18.00 (36.60) [1:18.00] 150m 1:58.50 (40.50) 200m 2:33.70 (35.20) [1:15.70] |
| 3 | Olgiata 20.12 SSD | Cangialosi Anna, Cundari Martina, Valentini Valeria, Fabbriconi Martina | 02'35''70 | 219 | | | |
50m 39.30 (39.30) 100m 1:17.70 (38.40) [1:17.70] 150m 1:59.10 (41.40) 200m 2:35.70 (36.60) [1:18.00] |
| 4 | I Swim Casetta Bianca SSD | Fiorelli Valeria, Funari Beatrice, Spiga Valeria, Cameo Sofia | 02'38''00 | 209 | | | |
50m 38.40 (38.40) 100m 1:19.30 (40.90) [1:19.30] 150m 2:00.70 (41.40) 200m 2:38.00 (37.30) [1:18.70] |
| 5 | Ippocampo SSD - Roma | Alessandrini Elisa, Piergiovanni Sofia Palmira, La Tegola Elisabetta, Iacovelli Sofia | 02'41''60 | 196 | | | |
50m 38.00 (38.00) 100m 1:17.70 (39.70) [1:17.70] 150m 2:01.50 (43.80) 200m 2:41.60 (40.10) [1:23.90] |
| | Coser Nuoto ASD | Capannini Federica, De Angelis Vanessa, Mori Maya, Gubiotti Chloe' | 02'41''60 | 196 | | | |
50m 42.90 (42.90) 100m 1:19.20 (36.30) [1:19.20] 150m 1:59.50 (40.30) 200m 2:41.60 (42.10) [1:22.40] |
| 7 | Sistemi Integrati Sport ASD - Roma | Silvestrini Sofia, Pettinella Flavia, Serafini Rebecca, Procopio Anna | 02'42''70 | 192 | | | |
50m 40.90 (40.90) 100m 1:20.80 (39.90) [1:20.80] 150m 2:01.50 (40.70) 200m 2:42.70 (41.20) [1:21.90] |
| 8 | Sport 2000 SSD - Roma | Modola Lavinia, De Luca Sofia, Medaglia Camilla, Collina Arianna | 02'44''90 | 184 | | | |
50m 38.60 (38.60) 100m 1:25.50 (46.90) [1:25.50] 150m 2:06.90 (41.40) 200m 2:44.90 (38.00) [1:19.40] |
| 9 | Athlon SSD - Roma | Principato Flavia, Manzo Camilla, Calderoni Flaminia, Sferrago Benedetta | 02'47''30 | 176 | | | |
50m 38.30 (38.30) 100m 1:20.70 (42.40) [1:20.70] 150m 2:06.60 (45.90) 200m 2:47.30 (40.70) [1:26.60] |
| 10 | Stelle Marine SSD | Trevisan Rachele, Mancini Elena, Cristofaro Federica, Di Nardo Eleonor | 02'50''60 | 166 | | | |
50m 43.70 (43.70) 100m 1:20.60 (36.90) [1:20.60] 150m 2:06.50 (45.90) 200m 2:50.60 (44.10) [1:30.00] |
| 11 | Green Hill ASD - Roma | Olivi Elisa, Piccardo Elisa, Greco Laura, Sandoval Torres Rebecca | 02'51''90 | 163 | | | |
50m 40.90 (40.90) 100m 1:25.40 (44.50) [1:25.40] 150m 2:10.30 (44.90) 200m 2:51.90 (41.60) [1:26.50] |
| 12 | Empire Roma SSD | Ferraro Marianna, Papi Alessia, De Berardinis Eleonora, Carlone Angela | 02'56''10 | 151 | | | |
50m 42.40 (42.40) 100m 1:26.50 (44.10) [1:26.50] 150m 2:11.60 (45.10) 200m 2:56.10 (44.50) [1:29.60] |
| 13 | Urbe Nuoto 90 | Graikhoksoong Sunee Nath, Bianchi Matilde, Caprini Francesca Maria, Talone Michela | 03'02''70 | 135 | | | |
50m 40.50 (40.50) 100m 1:27.20 (46.70) [1:27.20] 150m 2:12.50 (45.30) 200m 3:02.70 (50.20) [1:35.50] |
