| 1 | Hisham Osman Farida | 1995 | USA Pinnacle Racing | 00'26''32 | 799 |  |  | |
50m 26.32 (26.32) |
| 2 | Curzan Claire | 2004 | USA Tac Titans | 00'26''37 | 795 | | | |
50m 26.37 (26.37) |
| 3 | Kendall Amanda | 1991 | USA Mission Viejo Nadadores | 00'26''43 | 789 | | | |
50m 26.43 (26.43) |
| 4 | Dahlia Worrell Kelsi | 1994 | USA Cardinal Aquatics | 00'26''59 | 775 | | | |
50m 26.59 (26.59) |
| 5 | Schmidtke Aliena | 1992 | GER SC Magdeburg | 00'26''69 | 766 | | | |
50m 26.69 (26.69) |
| 6 | Hinds Natalie | 1993 | USA Athens Bulldog Swim Club | 00'26''78 | 759 | | | |
50m 26.78 (26.78) |
| 7 | Cuomo Lexi | 2001 | USA Mason Makos Swim Team | 00'27''56 | 696 | | | |
50m 27.56 (27.56) |
| 8 | Gingrich Leah | 1990 | USA Columbus Aquatic Club | 00'27''65 | 689 | | | |
50m 27.65 (27.65) |
| 9 | Kraus Alena | 1999 | USA University Of Louisville | 00'27''71 | 685 | | | |
50m 27.71 (27.71) |
| 10 | Trahan Erin K | 1997 | USA Southern Methodist University | 00'27''74 | 683 | | | |
50m 27.74 (27.74) |
| 11 | Kingsley Megan | 1996 | USA Athens Bulldog Swim Club | 00'27''75 | 682 | | | |
50m 27.75 (27.75) |
| 12 | Saiz Hannah | 1991 | USA Unattached SSTY | 00'27''76 | 681 | | | |
50m 27.76 (27.76) |
| 13 | Purcell Kayla | 1998 | USA Unattached | 00'27''80 | 678 | | | |
50m 27.80 (27.80) |
| 14 | Paez Isabella | 1995 | USA Pinnacle Racing | 00'27''86 | 674 | | | |
50m 27.86 (27.86) |
| 15 | Passon Felicity Elizabeth | 1999 | USA Southern Methodist University | 00'27''91 | 670 | | | |
50m 27.91 (27.91) |
| 16 | Atkinson Alia Shanee | 1988 | USA South Florida Aquatic Club | 00'28''16 | 652 | | | |
50m 28.16 (28.16) |
| 17 | D'Arcy Autumn | 2000 | USA Clovis Swim Club | 00'28''18 | 651 | | | |
50m 28.18 (28.18) |
| 18 | Skellett Alaina | 1999 | USA University Of Miami Hurricanes | 00'28''63 | 621 | | | |
50m 28.63 (28.63) |
| 19 | Mason Zorryonna | 2000 | USA University Of Miami Hurricanes | 00'28''77 | 612 | | | |
50m 28.77 (28.77) |
| 20 | Bruneau Julia | 1999 | USA Unattached | 00'28''79 | 611 | | | |
50m 28.79 (28.79) |
| 21 | Burton Meghan | 1998 | USA Unattached Gwu | 00'28''90 | 604 | | | |
50m 28.90 (28.90) |
| 22 | Burt Maddy | 2002 | USA Swim Florida | 00'28''96 | 600 | | | |
50m 28.96 (28.96) |
| 23 | Mcconagha Mackenzie | 2003 | USA Nation'S Capital Swim Club | 00'29''12 | 590 | | | |
50m 29.12 (29.12) |
| 24 | Graham Kayla | 2001 | USA Nation'S Capital Swim Club | 00'29''22 | 584 | | | |
50m 29.22 (29.22) |
| 25 | Whitlow Cabell | 2000 | USA Unattached | 00'29''26 | 582 | | | |
50m 29.26 (29.26) |
| 26 | Grossklaus Olivia | 2000 | USA Southern Methodist University | 00'29''66 | 558 | | | |
50m 29.66 (29.66) |
