| 1 | Polignano Chiara | 2001 | Airon Club Bio-Sport SSD | 00'33''50 | 619 |
| 2 | Castellaneta Sabrina | 2009 | Meridiana Nuoto Taranto SSD | 00'34''22 | 581 |
| 3 | Lampedecchia Mariacarla | 2006 | Netium SSD - Giovinazzo | 00'34''82 | 551 |
| 4 | Gloria Gaia | 2007 | Olimpica Salentina A.S.D. | 00'34''87 | 549 |
| 5 | Divella Giovanna | 2001 | Ranidae CSSD | 00'35''07 | 540 |
| 6 | Castellaneta Alessia | 2005 | Meridiana Nuoto Taranto SSD | 00'35''17 | 535 |
| 7 | Samarelli Federica | 2008 | Netium SSD - Giovinazzo | 00'35''50 | 520 |
| 8 | Grillo Giada | 2007 | Netium SSD - Giovinazzo | 00'35''76 | 509 |
| 9 | Salvadore Isa | 2006 | Olimpica Salentina A.S.D. | 00'35''84 | 506 |
| 10 | Farella Flavia | 2006 | Sports And Events SSD | 00'36''06 | 496 |
| 11 | Auletta Giada | 2009 | K-Sport Academy SSD | 00'37''31 | 448 |
| 12 | Schirone Angelica | 2007 | CUS Bari | 00'37''55 | 439 |
| 13 | Stufano Giuliamaria | 2010 | Netium SSD - Giovinazzo | 00'38''13 | 420 |
| 14 | Lato Alessia | 2009 | Olimpica Salentina A.S.D. | 00'38''21 | 417 |
| 15 | Rizzi Giulia | 2010 | Icos Sporting Club | 00'38''50 | 408 |
| 16 | Fino Fabiana | 2010 | CUS Bari | 00'38''78 | 399 |
| 17 | Giotta Flavia Lorenza Maria | 2006 | Olimpica Salentina A.S.D. | 00'38''86 | 396 |
| 18 | Valente Veronica | 2009 | CUS Bari | 00'38''97 | 393 |
| | Pidulo Ilaria | 2007 | Icos Sporting Club | 00'38''97 | 393 |
| 20 | Conte Matilde | 2008 | Airon Club Bio-Sport SSD | 00'39''36 | 382 |
| 21 | Minervini Flavia | 2008 | Netium SSD - Giovinazzo | 00'39''81 | 369 |
| 22 | Narracci Taisha | 2008 | Otre' SSD - Noci | 00'39''84 | 368 |
| 23 | Farina Anna | 2008 | Icos Sporting Club | 00'40''23 | 357 |
| 24 | Coppola Paola | 2009 | Miropa Fit SSD AR | 00'40''24 | 357 |
| 25 | Bellacicco Aurora | 2008 | Otre' SSD - Noci | 00'41''34 | 329 |
| 26 | Fumai Giulia | 2007 | CUS Bari | 00'41''74 | 320 |
| 27 | Depalma Matilde | 2009 | Netium SSD - Giovinazzo | 00'41''77 | 319 |
| 28 | Palmisano Elisabetta | 2008 | Icos Sporting Club | 00'42''35 | 306 |
| 29 | Tota Jennifer | 2010 | Team Puglia Scsd | 00'42''74 | 298 |
| 30 | Valerio Giorgia | 2009 | K-Sport Academy SSD | 00'43''21 | 288 |
| 31 | Vavallo Virginia | 2009 | Icos Sporting Club | 00'44''73 | 260 |
| 32 | De Carlo Elisa | 2008 | Albatros Sport Club SSD - Crispiano | 00'45''72 | 243 |
| 33 | Cappilli Chiara | 2007 | Muovi Lecce SSD | 00'45''81 | 242 |
| 34 | Angelini Aurora | 2010 | Aquatika SSD - Martina Franca | 00'46''17 | 236 |
| 35 | Meleleo Swami | 2010 | Olimpica Salentina A.S.D. | 00'49''68 | 189 |
