| 1 | Di Moia Ilaria | 2004 | Specialisti Dello Sport - L'Aquila | 00'28''70 | 709 |  |
| 2 | Rubino Giorgia | 2006 | Team Puglia Scsd | 00'29''22 | 672 |  |
| 3 | La Notte Alessandra | 2003 | Netium SSD - Giovinazzo | 00'30''16 | 611 |
| 4 | Testino Ilaria | 2007 | Team Puglia Scsd | 00'31''25 | 549 |
| 5 | Bruni Francesca Maria | 2006 | Netium SSD - Giovinazzo | 00'32''37 | 494 |
| 6 | Ritrovato Carolina | 2005 | CUS Bari | 00'32''93 | 469 |
| 7 | Putignano Gracia | 2006 | Meridiana Nuoto Taranto SSD | 00'33''12 | 461 |
| 8 | Porro Valeria | 2008 | Netium SSD - Giovinazzo | 00'33''13 | 461 |
| 9 | Galiano Gloria | 2003 | Meridiana Nuoto Taranto SSD | 00'33''43 | 449 |
| 10 | Cramarossa Giorgia | 2006 | CUS Bari | 00'33''53 | 445 |
| 11 | Gravili Laura | 2008 | Muovi Lecce SSD | 00'33''73 | 437 |
| 12 | Sergi Irene | 2006 | Meridiana Nuoto Taranto SSD | 00'33''76 | 436 |
| 13 | Terlizzi Claudia | 2008 | Sport Project SSD | 00'33''78 | 435 |
| 14 | Pierno Giulia | 2009 | Sport Project SSD | 00'33''94 | 429 |
| 15 | Duchetti Erika | 2008 | Meridiana Nuoto Taranto SSD | 00'34''01 | 426 |
| 16 | Borraccino Azzurra | 2006 | Meridiana Nuoto Taranto SSD | 00'34''02 | 426 |
| 17 | Cantisani Virginia | 2010 | K-Sport Academy SSD | 00'34''78 | 398 |
| 18 | Manca Mattea | 2008 | Icos Sporting Club | 00'34''82 | 397 |
| 19 | Genchi Roberta | 2006 | Sport Project SSD | 00'35''32 | 380 |
| 20 | Rodia Mariasofia | 2010 | Icos Sporting Club | 00'35''48 | 375 |
| 21 | Veneziani Sara | 2009 | Netium SSD - Giovinazzo | 00'36''22 | 353 |
| 22 | Trevisi Emma | 2007 | Team Puglia Scsd | 00'36''45 | 346 |
| 23 | Rizzo Sara | 2008 | Olimpica Salentina A.S.D. | 00'36''95 | 332 |
| 24 | Melpignano Camilla | 2010 | Olimpica Salentina A.S.D. | 00'36''96 | 332 |
| 25 | Cutrignelli Gaia | 2010 | K-Sport Academy SSD | 00'37''31 | 323 |
| 26 | Coppola Paola | 2009 | Miropa Fit SSD AR | 00'37''52 | 317 |
| 27 | Mignozzi Giada | 2009 | Miropa Fit SSD AR | 00'37''56 | 316 |
| | Ludovico Domenica | 2007 | Otre' SSD - Noci | 00'37''56 | 316 |
| 29 | Susca Ivana | 2006 | Airon Club Bio-Sport SSD | 00'37''62 | 315 |
| 30 | Palmisano Elisabetta | 2008 | Icos Sporting Club | 00'37''86 | 309 |
| 31 | Minardi Giulia | 2009 | Icos Sporting Club | 00'38''48 | 294 |
| 32 | Petrelli Sabrina | 2007 | K-Sport Academy SSD | 00'39''46 | 273 |
| 33 | Faccilongo Mariachiara | 2009 | Sport Project SSD | 00'39''91 | 263 |
| 34 | Gentile Mariantonietta | 2009 | Otre' SSD - Noci | 00'41''87 | 228 |
| 35 | De Carlo Elisa | 2008 | Albatros Sport Club SSD - Crispiano | 00'43''62 | 202 |
