| 1 | Nardi Paola | 2009 | Guidonia SC Manianpama ASD | 01'04''84 | 606 |  |
| 2 | Antonicelli Sonia | 2009 | Mediterraneo Sport SSD - Taranto | 01'06''22 | 569 |
| 3 | Travaglio Giorgia | 2009 | Racing Nuoto Roma SSD | 01'06''82 | 554 |
| 4 | Salerno Federica | 2010 | Polisportiva Balnaea - Battipaglia | 01'09''16 | 499 |
| 5 | Ichim Vanessa Ionela | 2009 | Centro Sport Roma SSD | 01'09''45 | 493 |
| 6 | Raimondi Emma | 2010 | Domar Sporting Club SSD | 01'10''48 | 472 |
| 7 | Cantisani Virginia | 2010 | K-Sport Academy SSD | 01'12''68 | 430 |
| 8 | Casini Claudia | 2009 | SSD FIN Plus Ostia | 01'14''79 | 395 |
| | Guariniello Roberta | 2009 | CUS Salerno | 01'14''79 | 395 |
| 10 | Maisto Ines | 2010 | Swim Academy SSD | 01'15''14 | 389 |
| 11 | Avantario Miriam | 2010 | K-Sport Academy SSD | 01'15''22 | 388 |
| 12 | Benedetti Carlotta | 2009 | Centro Sport Roma SSD | 01'15''53 | 383 |
| 13 | Binaghi Emma | 2009 | Effedue Group | 01'16''00 | 376 |
| 14 | Cappelli Matilda Pilar | 2009 | Effedue Group | 01'16''89 | 363 |
| 15 | Chessari Federica | 2010 | Centro Sport Roma SSD | 01'16''95 | 362 |
| 16 | Colantoni Matilde | 2010 | Stelle Marine SSD | 01'17''68 | 352 |
| 17 | Selvaggi Giulia | 2009 | Stelle Marine SSD | 01'17''85 | 350 |
| 18 | Natale Francesca | 2009 | Sport Village Fondi Nuoto SSD | 01'18''33 | 344 |
| 19 | Lancia Elisa | 2009 | Consorz. Le Cupole - Roma | 01'18''69 | 339 |
| 20 | Guglielmi Bianca | 2010 | Thebris Perugia | 01'18''77 | 338 |  |  | |
50m 37.74 (37.74) 100m 1:18.77 (41.03) [1:18.77] |
| 21 | Cutrignelli Gaia | 2010 | K-Sport Academy SSD | 01'19''67 | 327 | | | |
50m 38.88 (38.88) 100m 1:19.67 (40.79) [1:19.67] |
| 22 | Fiorillo Benedetta | 2009 | Polisportiva Balnaea - Battipaglia | 01'20''93 | 311 | | | |
50m 39.46 (39.46) 100m 1:20.93 (41.47) [1:20.93] |
| 23 | Villa Francesca | 2010 | Stelle Marine SSD | 01'21''50 | 305 | | | |
50m 39.34 (39.34) 100m 1:21.50 (42.16) [1:21.50] |
| 24 | Hyso Vittoria | 2010 | Thebris Perugia | 01'21''77 | 302 | | | |
50m 39.52 (39.52) 100m 1:21.77 (42.25) [1:21.77] |
| 25 | Della Ciana Flaminia | 2010 | Racing Nuoto Roma SSD | 01'22''30 | 296 | | | |
50m 40.45 (40.45) 100m 1:22.30 (41.85) [1:22.30] |
| 26 | Palmucci Valeria | 2010 | Centro Sport Roma SSD | 01'22''37 | 295 | | | |
50m 40.52 (40.52) 100m 1:22.37 (41.85) [1:22.37] |
| 27 | Capocci Eva | 2010 | Swim Academy SSD | 01'26''55 | 255 | | | |
50m 43.46 (43.46) 100m 1:26.55 (43.09) [1:26.55] |
| | Ricci Ludovica | 2009 | Heaven 2 SSD - Roma | Squalificato | |
