| 1 | Wasick Katarzyna | 1992 | USA Team Rebel Aquatics | 00'24''56 | 895 |
| 2 | Walsh Gretchen | 2003 | USA Nashville Aquatic Club | 00'24''65 | 885 |
| 3 | Weitzeil Abbigail | 1996 | USA California Aquatics | 00'24''97 | 851 |
| 4 | Albiero Gabriela | 2002 | USA University Of Louisville | 00'25''02 | 846 |
| 5 | Deloof Catherine | 1997 | USA New York Athletic Club | 00'25''05 | 843 |
| 6 | Countie Grace | 1999 | USA North Carolina Aquatic Club | 00'25''16 | 832 |
| 7 | O'Leary Mia | 2002 | AUS Australia | 00'25''24 | 824 |
| 8 | Parker Maxine | 2002 | USA Cavalier Swimming | 00'25''35 | 814 |
| 9 | Curzan Claire | 2004 | USA Tac Titans | 00'25''39 | 810 |
| 10 | Pelaez Erika | 2006 | USA Eagles Aquatics | 00'25''41 | 808 |
| | Fulmer Amy | 2001 | USA Ohio State University | 00'25''41 | 808 |
| 12 | Stepanek Chloe | 2002 | USA Long Island Aquatic Club | 00'25''45 | 804 |
| 13 | Johnson Kaitlyn | 1989 | USA Unattached | 00'25''56 | 794 |
| | Dupre Cora | 2001 | USA University Of Alabama | 00'25''56 | 794 |
| 15 | Flynn Lindsay | 2002 | USA Univ Of Michigan Swim Team | 00'25''58 | 792 |
| 16 | Regenauer Christiana | 2001 | USA University Of Louisville | 00'25''59 | 791 |
| 17 | Cooper Grace | 2002 | USA Unattached | 00'25''63 | 787 |
| 18 | Soma Ai | 1997 | USA Cardinal Aquatics | 00'25''64 | 786 |
| 19 | Semenuk Bridget | 2001 | USA Unattached | 00'25''67 | 784 |
| | Riley Eloise | 2001 | USA California Aquatics | 00'25''67 | 784 |
| | Scott Morgan | 2000 | USA University Of Alabama | 00'25''67 | 784 |
| 22 | Leibel Kyla | 2001 | USA Unattached | 00'25''72 | 779 |
| 23 | Winter Kailyn | 2003 | USA University Of Alabama | 00'25''78 | 774 |
| 24 | Kennedy Madison | 1987 | USA South Carolina Swim Club | 00'25''82 | 770 |
| 25 | Bates Talia | 2001 | USA Gator Swim Club | 00'25''85 | 767 |
| 26 | Wheal Emma | 2001 | USA Alto Swim Club | 00'25''87 | 765 |
| 27 | Welch Ella | 2004 | USA Cardinal Aquatics | 00'25''91 | 762 |
| 28 | Turak Ashley | 2000 | USA Indiana Swim Club | 00'25''93 | 760 |
| 29 | Webb Abbey | 2001 | AUS Australia | 00'25''96 | 758 |
| 30 | Nordmann Lillie | 2002 | USA Alto Swim Club | 00'26''00 | 754 |
| | Summit Maggie | 2001 | USA University of Minnesota | 00'26''00 | 754 |
| 32 | Zenick Katherine | 2001 | USA Ohio State University | 00'26''01 | 753 |
| | Ficken Lawson | 2004 | USA University Of Denver Hilltoppe | 00'26''01 | 753 |
| 34 | Ulett Tristen | 2002 | USA University Of Louisville | 00'26''05 | 750 |
| 35 | Yendell Sophie | 2002 | USA University Of Pittsburgh | 00'26''09 | 746 |
| | Jacobson Rebecca | 2002 | AUS Australia | 00'26''09 | 746 |
| 37 | Walsh Aislinn | 2000 | USA Ohio State University | 00'26''10 | 745 |
| | Mazurek Ella Marie | 2003 | USA Grand Island Ymca Quicksilver | 00'26''10 | 745 |
| 39 | Smith Sierra | 2002 | USA University Of Missouri | 00'26''12 | 744 |
| | Tang Amy Yawei | 2003 | USA Alto Swim Club | 00'26''12 | 744 |
| 41 | Cuomo Lexi | 2001 | USA Cavalier Swimming | 00'26''14 | 742 |
| 42 | Crom Katie | 2003 | USA Mission Viejo Nadadores | 00'26''15 | 741 |
| 43 | Dobler Kaitlyn | 2002 | USA Trojan Swim Club | 00'26''16 | 740 |
| 44 | Feddersen Amy | 2000 | USA University Of Missouri | 00'26''17 | 739 |
| 45 | Skirboll Zoe | 2004 | USA Racer X Aquatics | 00'26''20 | 737 |
| 46 | Salvino Teia Isabella | 2005 | USA Mission Viejo Nadadores | 00'26''22 | 735 |
| 47 | Mack Katie | 2001 | USA Gator Swim Club | 00'26''23 | 734 |
| 48 | Milutinovich Katarina | 2001 | USA Lsu Swimming | 00'26''27 | 731 |
| 49 | Howley Tess | 2005 | USA Long Island Aquatic Club | 00'26''29 | 729 |
| 50 | Garcia Elise | 2000 | USA Trojan Swim Club | 00'26''31 | 728 |
| 51 | Ivanova Teresa | 2003 | USA Ohio State University | 00'26''34 | 725 |
| 52 | Shaw Anna | 2002 | USA Unattached | 00'26''40 | 720 |
| 53 | Mulvihill Lexie | 2003 | USA Auburn University Swim Club | 00'26''44 | 717 |
| 54 | Steckiel Emma | 2002 | USA Auburn University Swim Club | 00'26''45 | 716 |
| 55 | Brito Bella | 2007 | USA Beach Cities Swimming | 00'26''48 | 714 |
| 56 | Sticklen Emma | 2002 | USA Unattached | 00'26''56 | 707 |
| 57 | Kan Cheuk Tung Natalie | 2002 | USA Univ Of Michigan Swim Team | 00'26''60 | 704 |
| 58 | Smith Skyler | 2003 | USA North Carolina Aquatic Club | 00'26''61 | 703 |
| 59 | Kern Emma | 2005 | USA Aquajets Swim Team | 00'26''79 | 689 |
| 60 | Greenhawt Lexi | 2005 | USA University Of Denver Hilltoppe | 00'27''31 | 651 |
| | Newman Claire | 2002 | USA Univ Of Michigan Swim Team | Squalificato | |
