| 1 | Arcudi Emma | 2009 | Pianeta Sport ASD - Reggio Calabria | 00'29''48 | 517 |
| 2 | D'Agata Carlotta Rita Ma | 2008 | Circolo Canottieri Ortigia 1928 | 00'29''85 | 498 |
| 3 | Arancio Elena | 2008 | Aqua Fit SSD - San Gregorio | 00'30''37 | 473 |
| 4 | Ferrara Sabrina Maria | 2009 | Pianeta Sport ASD - Reggio Calabria | 00'30''81 | 453 |
| 5 | Cacciola Nicoletta | 2008 | Circolo Canottieri Ortigia 1928 | 00'31''48 | 425 |
| 6 | Valenti Ivana | 2008 | Poseidon Swim SSD | 00'31''61 | 419 |
| 7 | Cuzzupe' Lucia | 2008 | Tennis Club Match Ball ASD - Siracusa | 00'31''91 | 408 |
| 8 | Nobile Chiara | 2008 | Nuoto Chiaramonte ASD | 00'32''00 | 404 |
| 9 | Pentecoste Carlotta | 2009 | Tennis Club Match Ball ASD - Siracusa | 00'32''32 | 392 |
| 10 | Stracuzzi Teresa | 2009 | Unime SSD - Messina | 00'32''43 | 388 |
| 11 | Bruno Margherita | 2008 | Tennis Club Match Ball ASD - Siracusa | 00'32''60 | 382 |
| 12 | Zappala' Marta Maria | 2009 | Aqua Fit SSD - San Gregorio | 00'32''61 | 382 |
| 13 | Randazzo Giordana | 2008 | Poseidon Swim SSD | 00'32''73 | 378 |
| 14 | Costantino Aurora Pia | 2008 | Swimblu ASD - Milazzo | 00'33''02 | 368 |
| 15 | Capurro Carlotta | 2008 | Pianeta Sport ASD - Reggio Calabria | 00'33''12 | 365 |
| 16 | Pluchino Francesca | 2009 | Nuoto Chiaramonte ASD | 00'33''13 | 364 |
| 17 | Di Giorgi Chiara | 2008 | Water Sun Comiso ASD | 00'33''18 | 363 |
| 18 | D'Amico Matilda | 2008 | Water Sun Comiso ASD | 00'33''26 | 360 |
| 19 | Zappala' Linda | 2009 | Tennis Club Match Ball ASD - Siracusa | 00'33''28 | 359 |
| 20 | Zenzaro Marta | 2008 | Water Sun Comiso ASD | 00'33''50 | 352 |
| 21 | Damiano Gaia | 2009 | Tennis Club Match Ball ASD - Siracusa | 00'34''00 | 337 |
| 22 | Privitera Sofia Elvira | 2009 | Poseidon Swim SSD | 00'34''26 | 329 |
| 23 | Battaglia Aurora | 2008 | Athon ASD - Augusta | 00'34''57 | 320 |
| 24 | Mangano Silvia | 2009 | Unime SSD - Messina | 00'34''71 | 317 |
| 25 | Durante Giulia | 2008 | Athon ASD - Augusta | 00'35''13 | 305 |
| 26 | Ragusa Lorena | 2008 | Poseidon Swim SSD | 00'35''71 | 291 |
| 27 | Arbitrio Anna | 2009 | Pianeta Sport ASD - Reggio Calabria | 00'35''78 | 289 |
| 28 | Marletta Alice | 2009 | Polisportiva Muri Antichi - Catania | 00'36''11 | 281 |
| 29 | Leone Lucrezia | 2009 | Unime SSD - Messina | 00'36''15 | 280 |
| 30 | Calamoneri Viola | 2009 | Unime SSD - Messina | 00'36''56 | 271 |
| 31 | Sturniolo Chiara | 2008 | Swimblu ASD - Milazzo | 00'37''33 | 254 |
| 32 | Salamone Cecilia | 2009 | Athon ASD - Augusta | 00'39''31 | 218 |
| 33 | Tripoli Lucrezia | 2009 | Athon ASD - Augusta | 00'39''37 | 217 |
