| 1 | Douglass Katherine | 2001 | USA New York Athletic Club | 00'24''38 | 915 |
| 2 | Huske Torri | 2002 | USA Arlington Aquatic Club | 00'24''41 | 911 |
| | Weitzeil Abbigail | 1996 | USA California Aquatics | 00'24''41 | 911 |
| 4 | Walsh Gretchen | 2003 | USA University Of Virginia | 00'24''42 | 910 |
| 5 | Haughey Siobhan Bernadette | 1997 | HKG Hong Kong | 00'24''62 | 888 |
| 6 | Henique Melanie | 1992 | FRA CN Marseille | 00'24''76 | 873 |
| 7 | Manuel Simone | 1996 | USA Sun Devil Swimming | 00'24''82 | 867 |
| 8 | Curzan Claire | 2004 | USA Tac Titans | 00'24''92 | 856 |
| 9 | Paegle Kristina | 2004 | USA Indiana University | 00'24''93 | 855 |
| 10 | Smoliga Olivia | 1994 | USA Sun Devil Swimming | 00'24''99 | 849 |
| 11 | Cooper Grace | 2002 | USA Texas A & M University | 00'25''10 | 838 |
| 12 | Moesch Anna | 2005 | USA Greater Somerset County Ymca S | 00'25''16 | 832 |
| 13 | Gaspard Florine | 2001 | FRA CN Marseille | 00'25''19 | 829 |
| 14 | Parker Maxine | 2002 | USA University Of Virginia | 00'25''23 | 825 |
| 15 | Larsen Caroline | 2006 | USA Foxjets Swim Team | 00'25''33 | 815 |
| 16 | Dennis Julia | 2004 | USA University Of Louisville | 00'25''40 | 809 |
| 17 | Spink Camille | 2004 | USA University Of Tennessee | 00'25''43 | 806 |
| 18 | Nel Olivia | 2002 | USA University Of North Carolina | 00'25''48 | 801 |
| | Erisman Rylee | 2009 | USA Laker Swim | 00'25''48 | 801 |
| 20 | Hisham Osman Farida | 1995 | USA California Aquatics | 00'25''51 | 798 |
| 21 | Fulmer Amy | 2001 | USA Ohio State University | 00'25''55 | 795 |
| 22 | Sanchez Noelle Kayla | 2001 | USA Mission Viejo Nadadores | 00'25''62 | 788 |
| 23 | Connolly Erika | 1998 | USA Tennessee Aquatics | 00'25''77 | 774 |
| 24 | Pelaez Erika | 2006 | USA Eagles Aquatics | 00'25''95 | 758 |
