| 1 | Weitzeil Abbigail | 1996 | USA California Aquatics | 00'24''24 | 931 |
| 2 | Walsh Gretchen | 2003 | USA Nashville Aquatic Club | 00'24''53 | 898 |
| 3 | Douglass Katherine | 2001 | USA New York Athletic Club | 00'24''57 | 894 |
| 4 | Deloof Catherine | 1997 | USA New York Athletic Club | 00'24''71 | 878 |
| 5 | Brown Erika | 1998 | USA Tennessee Aquatics | 00'24''91 | 857 |
| 6 | Smoliga Olivia | 1994 | USA Sun Devil Swimming | 00'24''96 | 852 |
| 7 | Albiero Gabriela | 2002 | USA University Of Louisville | 00'24''98 | 850 |
| 8 | Huske Torri | 2002 | USA Arlington Aquatic Club | 00'25''02 | 846 |
| 9 | Cooper Grace | 2002 | USA Longhorn Aquatics | 00'25''13 | 835 |
| | Curzan Claire | 2004 | USA Alto Swim Club | 00'25''13 | 835 |
| 11 | Parker Maxine | 2002 | USA Cavalier Swimming | 00'25''16 | 832 |
| 12 | Fulmer Amy | 2001 | USA Ohio State University | 00'25''18 | 830 |
| 13 | Berkoff Katharine France | 2001 | USA Wolfpack Elite | 00'25''31 | 817 |
| 14 | Turak Ashley | 2000 | USA Indiana Swim Club | 00'25''32 | 816 |
| 15 | Paegle Kristina | 2004 | USA Indiana Swim Club | 00'25''35 | 814 |
| | Larsen Caroline | 2006 | USA Foxjets Swim Team | 00'25''35 | 814 |
| 17 | Dennis Julia | 2004 | USA University Of Louisville | 00'25''36 | 813 |
| 18 | Olasewere Annam | 2007 | GBR Chelsea Piers Aquatic Club | 00'25''37 | 812 |
| | Regenauer Christiana | 2001 | USA University Of Louisville | 00'25''37 | 812 |
| 20 | Moesch Anna | 2005 | USA Greater Somerset County Ymca S | 00'25''38 | 811 |
| 21 | Stepanek Chloe | 2002 | USA Long Island Aquatic Club | 00'25''44 | 805 |
| | Gemmell Erin | 2004 | USA Nation'S Capital Swim Club | 00'25''44 | 805 |
| 23 | Tierney Hailey | 2005 | USA Lake Country Swim Team | 00'25''50 | 799 |
| 24 | Hinds Natalie | 1993 | USA Gator Swim Club | 00'25''53 | 796 |
| 25 | Christianson Lily | 2006 | USA Irish Aquatics | 00'25''54 | 796 |
| 26 | Pelaez Erika | 2006 | USA Eagles Aquatics | 00'25''55 | 795 |
| 27 | Pash Kelly | 2001 | USA Longhorn Aquatics | 00'25''58 | 792 |
| 28 | Zenick Katherine | 2001 | USA Ohio State University | 00'25''62 | 788 |
| | Shackell Alex | 2006 | USA Carmel Swim Club | 00'25''62 | 788 |
| 30 | Newman Claire | 2002 | USA Univ Of Michigan Swim Team | 00'25''70 | 781 |
| 31 | Spink Camille | 2004 | USA Nation'S Capital Swim Club | 00'25''75 | 776 |
| 32 | Tang Amy Yawei | 2003 | USA Alto Swim Club | 00'25''79 | 773 |
| 33 | King Lily | 2007 | USA Mount Pleasant Swim Club | 00'25''80 | 772 |
| 34 | Johnson Kaitlyn | 1989 | USA Club Mountaineer Aquatics | 00'25''83 | 769 |
| 35 | Sims Arabella | 2005 | USA Sandpipers Of Nevada | 00'25''86 | 766 |
| | Melilli Danielle | 2000 | USA Queens University | 00'25''86 | 766 |
| 37 | Karl Avery | 2006 | USA Empire Swimming | 00'25''87 | 765 |
| 38 | Flynn Lindsay | 2002 | USA Univ Of Michigan Swim Team | 00'25''88 | 765 |
| 39 | Wanner Olivia | 2006 | USA Waukesha Express Swim Team | 00'25''89 | 764 |
| 40 | Connelly Josie | 2006 | USA Oly Swimming | 00'25''92 | 761 |
| 41 | Longi Ava | 2002 | USA Longhorn Aquatics | 00'25''94 | 759 |
| 42 | Scott Morgan | 2000 | USA Texas Ford Aquatics | 00'25''97 | 757 |
| 43 | Mccarty Eboni | 2003 | USA Athens Bulldog Swim Club | 00'25''98 | 756 |
| 44 | Sauickie Addison | 2006 | USA Sarasota Ymca Sharks | 00'26''01 | 753 |
| 45 | Ervin Lindsay | 2004 | USA Irvine Novaquatics | 00'26''05 | 750 |
| 46 | Fulton Alexa | 2004 | USA Upper Main Line Ymca | 00'26''07 | 748 |
| 47 | Peplowski Anna | 2002 | USA Indiana Swim Club | 00'26''09 | 746 |
| 48 | Davidson Emma | 2001 | USA Longhorn Aquatics | 00'26''13 | 743 |
| 49 | Kendall Brady | 2004 | USA Univ Of Michigan Swim Team | 00'26''15 | 741 |
| 50 | Nesty Lillie A | 2006 | USA Gator Swim Club | 00'26''18 | 739 |
| 51 | Mulvihill Lexie | 2003 | USA Auburn University Swim Club | 00'26''20 | 737 |
| 52 | Cronk Micayla | 2003 | USA University Of Florida | 00'26''25 | 733 |
| 53 | Welch Ella | 2004 | USA University Of Louisville | 00'26''27 | 731 |
| 54 | Russo Catherine | 2002 | USA Ohio State University | 00'26''37 | 723 |
| 55 | Kennedy Madison | 1987 | USA South Carolina Swim Club | 00'26''42 | 719 |
| 56 | Stephens Julianna | 2001 | USA Swimatlanta | 00'26''45 | 716 |
| 57 | Crush Charlotte | 2008 | USA Lakeside Swim Team | 00'26''55 | 708 |
| 58 | Funderburke Nyah | 2003 | USA Ohio State University | 00'26''57 | 707 |
| 59 | Kern Emma | 2005 | USA Aquajets Swim Team | 00'26''63 | 702 |
| 60 | Dobler Kaitlyn | 2002 | USA Trojan Swim Club | 00'26''68 | 698 |
| 61 | Sikes Katie Belle | 2005 | USA East Carolina Aquatics | 00'26''77 | 691 |
