| 1 | Azzurra Race Team | Luca' Davide, Tanci Chiara, Piorigo Mattia, Sordini Sofia | 02'19''30 | 329 |  |  | |
50m 34.70 (34.70) 100m 1:11.60 (36.90) [1:11.60] 150m 1:42.90 (31.30) 200m 2:19.30 (36.40) [1:07.70] |
| 2 | Gryphus Sporting Club | Rosati Paolo, Carli Vittoria, Mucci Alessandro, Tini Giorgia | 02'21''80 | 312 | | | |
50m 35.10 (35.10) 100m 1:11.10 (36.00) [1:11.10] 150m 1:41.20 (30.10) 200m 2:21.80 (40.60) [1:10.70] |
| 3 | Thebris Perugia | Geoli Nicola, Nuzzo Giorgio, Antoniucci Giorgia, Ceccarelli Letizia | 02'29''30 | 267 | | | |
50m 37.00 (37.00) 100m 1:14.00 (37.00) [1:14.00] 150m 1:53.10 (39.10) 200m 2:29.30 (36.20) [1:15.30] |
| 4 | Ternana Nuoto ASD | Gennari Filippo, Leone Francesca, Rondolini Veronica, Polara Marco | 02'31''20 | 257 | | | |
50m 34.60 (34.60) 100m 1:16.10 (41.50) [1:16.10] 150m 1:55.90 (39.80) 200m 2:31.20 (35.30) [1:15.10] |
| 5 | Gryphus Sporting Club | Fulle Giovanni, Balinca Raul Vasile, Lupinelli Chiara, Rossi Terradura Anna | 02'36''20 | 233 | | | |
50m 37.40 (37.40) 100m 1:29.20 (51.80) [1:29.20] 150m 1:59.20 (30.00) 200m 2:36.20 (37.00) [1:07.00] |
| 6 | Polisport SRL - Città Di Castello | Cenci Elisa, Cardinali Rachele, Gregori Riccardo, Mercati Matteo | 02'36''40 | 232 | | | |
50m 41.30 (41.30) 100m 1:22.30 (41.00) [1:22.30] 150m 2:00.50 (38.20) 200m 2:36.40 (35.90) [1:14.10] |
| 7 | Azzurra Race Team | Valentini Saverio, Domenichini Asia, Gagliardi Cristian, Brozzetti Maria | 02'38''10 | 225 | | | |
50m 38.10 (38.10) 100m 1:18.40 (40.30) [1:18.40] 150m 2:02.10 (43.70) 200m 2:38.10 (36.00) [1:19.70] |
| 8 | Gryphus Sporting Club | Mattiacci Maria Vittoria, Zidani Ayoub, Trejo Picon Dylan Mathias, Pisante Anna Giulia | 02'44''60 | 199 | | | |
50m 38.80 (38.80) 100m 1:22.80 (44.00) [1:22.80] 150m 2:03.60 (40.80) 200m 2:44.60 (41.00) [1:21.80] |
| 9 | Thebris Perugia | Carelli Matteo, Virelles Aguila Leonardo, Vincenti Arianna, Fiorucci Matilde | 02'46''20 | 193 | | | |
50m 38.80 (38.80) 100m 1:20.50 (41.70) [1:20.50] 150m 2:04.10 (43.60) 200m 2:46.20 (42.10) [1:25.70] |
| 10 | Planet Sport Todi SSD | Granieri Tommaso, Pagliaccia Giorgia, Cinti Edoardo, Posti Matilde | 02'50''60 | 179 | | | |
50m 41.10 (41.10) 100m 1:27.40 (46.30) [1:27.40] 150m 2:08.50 (41.10) 200m 2:50.60 (42.10) [1:23.20] |
| 11 | Village SSD - Todi | Passeri Viola, Tenerini Anna, Pignattini Lorenzo, Pettinari Mattia | 02'51''20 | 177 | | | |
50m 45.50 (45.50) 100m 1:28.60 (43.10) [1:28.60] 150m 2:08.30 (39.70) 200m 2:51.20 (42.90) [1:22.60] |
| 12 | Azzurra Race Team | Baldelli Vittorio, Truffelli Noemi, Caporalini Andrea, Mirti Vittoria | 03'10''40 | 128 | | | |
50m 43.30 (43.30) 100m 1:15.70 (32.40) [1:15.70] 150m 2:11.50 (55.80) 200m 3:10.40 (58.90) [1:54.70] |
| 13 | Azzurra Race Team | Marcantonini Tommaso, Sigismondi Bianca, Imbroglia Filippo, Vitalesta Marialuisa | 03'14''20 | 121 | | | |
50m 47.70 (47.70) 100m 1:40.10 (52.40) [1:40.10] 150m 2:27.40 (47.30) 200m 3:14.20 (46.80) [1:34.10] |
| 14 | Polisport SRL - Città Di Castello | Arib Wardia Katia, Bardassini Linda, Imperato Gennaro, Monaldi Gabriele | 03'35''20 | 89 | | | |
50m 1:05.50 (1:05.50) 100m 1:53.30 (47.80) [1:53.30] 150m 2:51.00 (57.70) 200m 3:35.20 (44.20) [1:41.90] |
