| 1 | Polisportiva Mimmo Ferrito SSD | Giordano Angelica, Lindiner Beatrice, Laquidara Sara, La Corte Mariaelena | 02'20''83 | 322 |  |  | |
50m 1:08.43 (1:08.43) 100m 2:20.83 (1:12.40) [2:20.83] |
| 2 | Sun Club SSD - Siracusa | Lentini Giuliana, Mudano' Lucia, Papa Aurora, Moceo Giorgia | 02'21''81 | 316 | | | |
50m 1:11.16 (1:11.16) 100m 2:21.81 (1:10.65) [2:21.81] |
| 3 | CUS Palermo | Mammina Sofia, Gentile Adele, Schillaci Giulia, Manni Virginia | 02'24''32 | 299 | | | |
50m 1:13.04 (1:13.04) 100m 2:24.32 (1:11.28) [2:24.32] |
| 4 | Poseidon Sporting Club SSD - Catania | Timpanaro Martina Maria, Errigo Benedetta, Caselli Delia, Arancio Gaia | 02'26''43 | 287 | | | |
50m 1:13.26 (1:13.26) 100m 2:26.43 (1:13.17) [2:26.43] |
| 5 | Polisportiva Mimmo Ferrito SSD | Severino Sofia, Rappa Emilia, Aversa Aurora, Cernigliaro Miryam | 02'27''38 | 281 | | | |
50m 1:12.74 (1:12.74) 100m 2:27.38 (1:14.64) [2:27.38] |
| 6 | Altair Nuoto SSD - Catania | Catania Anita, Napolitano Desiree, Greco Giada, Rua' Elisabetta | 02'27''47 | 281 | | | |
50m 1:13.85 (1:13.85) 100m 2:27.47 (1:13.62) [2:27.47] |
| 7 | Polisportiva Sport Club Erea ASD | Pluchino Francesca, Schembari Laetitia, Salvo Rossi Carla, Iacono Marisol | 02'35''86 | 238 | | | |
50m 1:16.29 (1:16.29) 100m 2:35.86 (1:19.57) [2:35.86] |
| 8 | Tennis Club Match Ball ASD - Siracusa | Damiano Gaia, Ciotta Stephany, Zappala' Linda, Pentecoste Carlotta | 02'36''30 | 236 | | | |
50m 1:18.55 (1:18.55) 100m 2:36.30 (1:17.75) [2:36.30] |
| 9 | Neri Fitness Club ASD | Troncia Jennifer, Troncia Jessica, Riccardi Serena, La Galia Martina | 02'37''00 | 233 | | | |
50m 1:18.84 (1:18.84) 100m 2:37.00 (1:18.16) [2:37.00] |
| 10 | Power Team Messina | Lamalfa Roberta, Cundari Emanuela, Mangano Silvia, Cucinotta Sonia | 02'40''05 | 219 | | | |
50m 1:18.99 (1:18.99) 100m 2:40.05 (1:21.06) [2:40.05] |
| 11 | Aquarius ASD - Trapani | Gavini Edvige, Pietrarossa Emanuela, Trapani Monika, Virgilio Greta | 02'41''29 | 214 | | | |
50m 1:21.72 (1:21.72) 100m 2:41.29 (1:19.57) [2:41.29] |
| 12 | Poseidon Sporting Club SSD - Catania | Papale Vittoria, Dellutri Sara, Puglisi Giulia, Costanzo Ginevra | 02'43''70 | 205 | | | |
50m 1:23.49 (1:23.49) 100m 2:43.70 (1:20.21) [2:43.70] |
| 13 | Polisportiva Nadir - Palermo | Scozzola Sofia, Calisti Andrea, Di Bella Eleonora, Vaccarino Aurora | 02'46''77 | 194 | | | |
50m 1:24.40 (1:24.40) 100m 2:46.77 (1:22.37) [2:46.77] |
| 14 | Waterpolo Messina ASD | Bonina Chiara, Noe' Ginevra Annagreta, Barbaro Rebecca, Irrera Angelica | 02'47''14 | 193 | | | |
50m 1:22.20 (1:22.20) 100m 2:47.14 (1:24.94) [2:47.14] |
| 15 | Unime SSD - Messina | Ribecco Maria Pia, Caselli Ludovica, Calamoneri Viola, Caristi Giulia | 02'54''99 | 168 | | | |
50m 1:24.95 (1:24.95) 100m 2:54.99 (1:30.04) [2:54.99] |
| 16 | Torre Del Grifo Village | Scuderi Miriam, Pulvirenti Ludovica, Pappalardo Annachiara Michela, Privitera Sofia Elvira | 02'57''49 | 161 | | | |
50m 1:26.76 (1:26.76) 100m 2:57.49 (1:30.73) [2:57.49] |
| 17 | Marsala Nuoto ASD | Maggio Simona, Rallo Federica, Genna Giorgia Anna, Frazzitta Carla | 03'00''37 | 153 | | | |
50m 1:18.52 (1:18.52) 100m 3:00.37 (1:41.85) [3:00.37] |
