| 1 | Aquatica Torino S.S.D.a.r.l. | Roletto Elisa, Pislor Marta, Bassi Emma, Venturi Ginevra Anna | 02'10''56 | 376 |  |  | |
50m 32.25 (32.25) 50m 2:10.56 (1:38.31) |
| 2 | UISP Vivisport | Monge Veronica, Reggio Giulia, Freilino Vittoria, Masuello Beatrice | 02'11''10 | 371 | | | |
50m 31.45 (31.45) 50m 2:11.10 (1:39.65) |
| 3 | Villa Becchi Nuoto ASD | Sogno Clara, Barcellini Camilla, Ghannadzadeh Shila, Musati Giada | 02'12''48 | 359 | | | |
50m 32.90 (32.90) 50m 2:12.48 (1:39.58) |
| 4 | Centro Sportivo Collegio San Giuseppe ASD | Trogu Giovanna, Verdun Benedetta, Spoto Matilde, Cazzola Arianna | 02'14''33 | 345 | | | |
50m 32.88 (32.88) 50m 2:14.33 (1:41.45) |
| 5 | Nuoto Club Alessandria | Sciutto Camilla, Scozzaro Martina, Iglina Giorgia, Prevignano Julia Federica Maria | 02'18''50 | 315 | | | |
50m 33.78 (33.78) 50m 2:18.50 (1:44.72) |
| 6 | UISP River Borgaro | Vigna Fabiola, Meriano Martina, Sama' Chiara | 02'19''10 | 310 | | | |
50m 33.76 (33.76) 50m 2:19.10 (1:45.34) |
| FG | Villa Becchi Nuoto ASD | Bertona Giulia, Siviero Marta, Botta Elena, Sogno Clara | 02'21''23 | 297 | | | |
50m 33.49 (33.49) 50m 2:21.23 (1:47.74) |
