| 1 | Olimpic Nuoto Napoli | Mallardo Alessandro, Santoro Delia, Capuozzo Loris, Cacciapuoti Marina | 01'56''09 | |  |  | |
50m 28.54 (28.54) 100m 57.12 (28.58) [57.12] 150m 1:26.80 (29.68) 200m 1:56.09 (29.29) [58.97] |
| 2 | Ippocampo SSD - Roma | Magrini Marco, Bernardinetti Maria, Giovannetti Giulia, Smimmo Francesco | 01'59''21 | | | | |
50m 28.68 (28.68) 100m 1:00.99 (32.31) [1:00.99] 150m 1:31.48 (30.49) 200m 1:59.21 (27.73) [58.22] |
| 3 | Accademia Nuoto Caserta | Fusco Raffaele, Vacchio Denise, De Cristofaro Sara, De Simone Raffaele | 01'59''23 | | | | |
50m 29.02 (29.02) 100m 1:00.18 (31.16) [1:00.18] 150m 1:31.80 (31.62) 200m 1:59.23 (27.43) [59.05] |
| 4 | Larus Nuoto ASD - Roma | Bianchi Angelica, Betti Alessio, Iapilone Ilaria, Cascino Gabriele | 02'02''21 | | | | |
50m 32.01 (32.01) 100m 1:01.09 (29.08) [1:01.09] 150m 1:32.46 (31.37) 200m 2:02.21 (29.75) [1:01.12] |
| 5 | Acquatix Atleticos Pompei | Russo Lucia, Pagano Giuseppe Francesco, Castaldi Giusy, Visciano Mario | 02'05''13 | | | | |
50m 31.66 (31.66) 100m 1:02.80 (31.14) [1:02.80] 150m 1:34.51 (31.71) 200m 2:05.13 (30.62) [1:02.33] |
| 6 | New Olimpic SSD - Bari | Ingravallo Federica, Lorusso Martina, Consonni Daniele, Natuzzi Andrea | 02'05''20 | | | | |
50m 31.18 (31.18) 100m 1:03.25 (32.07) [1:03.25] 150m 1:33.51 (30.26) 200m 2:05.20 (31.69) [1:01.95] |
| 7 | Gym Line SRL SSD | Faina Christian, Amati Sofia, Montanini Valerio, Andreucci Diana | 02'06''40 | | | | |
50m 30.05 (30.05) 100m 1:01.14 (31.09) [1:01.14] 150m 1:33.60 (32.46) 200m 2:06.40 (32.80) [1:05.26] |
| 8 | Olimpic Nuoto Napoli | Avallone Mariano, Capuozzo Mattia, Marino Susanna, Carmicino Eleonora | 02'07''26 | | | | |
50m 31.85 (31.85) 100m 1:03.19 (31.34) [1:03.19] 150m 1:35.75 (32.56) 200m 2:07.26 (31.51) [1:04.07] |
| 9 | Larus Nuoto ASD - Roma | Buzdugan Ilaria, Berini Federico, Di Domenico Sara, Paglioni Emanuele | 02'07''51 | | | | |
50m 35.30 (35.30) 100m 1:05.34 (30.04) [1:05.34] 150m 1:38.27 (32.93) 200m 2:07.51 (29.24) [1:02.17] |
| 10 | Fritz Dennerlein ASD | Carrotta Luana, Sannino Angelapiera, Del Mastro Ciro Antonio, Forcelli Michele | 02'08''86 | | | | |
50m 31.18 (31.18) 100m 1:04.24 (33.06) [1:04.24] 150m 1:36.27 (32.03) 200m 2:08.86 (32.59) [1:04.62] |
| 11 | Splash ASD - Potenza | Vertone Giuseppe, Mucciarone Elena, Grieco Roberta, Vignola Michele | 02'09''13 | | | | |
50m 32.24 (32.24) 100m 1:05.66 (33.42) [1:05.66] 150m 1:40.24 (34.58) 200m 2:09.13 (28.89) [1:03.47] |
| 12 | Atleti Amatori Matera SSD | Salinari Biagio, Massa Francesca, Catena Martina, Palazzo Francesco | 02'10''71 | | | | |
50m 33.26 (33.26) 100m 1:04.43 (31.17) [1:04.43] 150m 1:36.06 (31.63) 200m 2:10.71 (34.65) [1:06.28] |
| 13 | Accademia Nuoto Caserta | Pascarella Marco, Girardi Chiara, Frungillo Francesca, Mannillo Marco | 02'10''75 | | | | |
50m 33.25 (33.25) 100m 1:06.51 (33.26) [1:06.51] 150m 1:39.18 (32.67) 200m 2:10.75 (31.57) [1:04.24] |
| 14 | AICS Nuoto Potenza ASD | Onorati Vito, Coiro Sofia, Pisano Anna, Diano' Marco Michele | 02'12''51 | | | | |
50m 30.81 (30.81) 100m 1:06.78 (35.97) [1:06.78] 150m 1:42.14 (35.36) 200m 2:12.51 (30.37) [1:05.73] |
| 15 | Funsport ASD - Roma | Corgnale Massimo, Spiroski Marta, Isoni Alessandro, Bucciarelli Aurora | 02'13''45 | | | | |
50m 33.56 (33.56) 100m 1:05.17 (31.61) [1:05.17] 150m 1:39.01 (33.84) 200m 2:13.45 (34.44) [1:08.28] |
| 16 | Vanessa Nuoto Smile ASD | Zotti Andrea, Cafiero Carlotta, Ricci Andrea Rocco, Capitanio Martina | 02'13''85 | | | | |
50m 30.70 (30.70) 100m 1:03.62 (32.92) [1:03.62] 150m 1:37.62 (34.00) 200m 2:13.85 (36.23) [1:10.23] |
| 17 | Accademia Nuoto Caserta | Buffolino Giuseppe, De Simone Federica, Zona Benedetta, Pengue Camillo | 02'13''90 | | | | |
50m 32.41 (32.41) 100m 1:06.91 (34.50) [1:06.91] 150m 1:41.68 (34.77) 200m 2:13.90 (32.22) [1:06.99] |
| 18 | Gym Line SRL SSD | Gregoletto Gaia, Calligari Alessandra, Boccuccia Tommaso, Pacini Leonardo | 02'14''11 | | | | |
50m 32.98 (32.98) 100m 1:07.35 (34.37) [1:07.35] 150m 1:40.33 (32.98) 200m 2:14.11 (33.78) [1:06.76] |
| 19 | Splash ASD - Potenza | Collazzo Giovanni, Salvia Simone Vincenzo, Lauletta Melania Domenica, Collazzo Aurora | 02'15''51 | | | | |
50m 34.33 (34.33) 100m 1:07.58 (33.25) [1:07.58] 150m 1:41.29 (33.71) 200m 2:15.51 (34.22) [1:07.93] |
| 20 | Dream Team Salerno | Antinolfi Marina, Bernardo Carmine, Esposito Sara, Lauri Andrea | 02'16''98 | | | | |
50m 34.68 (34.68) 100m 1:08.24 (33.56) [1:08.24] 150m 1:45.07 (36.83) 200m 2:16.98 (31.91) [1:08.74] |
| 21 | Feel Good SSD - Cellino San Marco | Indirli Filippo, De Marco Alessia, Casilli Agnese, Marseglia Jacopo | 02'18''69 | | | | |
50m 34.11 (34.11) 100m 1:11.34 (37.23) [1:11.34] 150m 1:45.62 (34.28) 200m 2:18.69 (33.07) [1:07.35] |
| 22 | New Olimpic SSD - Bari | Palattella Alessia Rita, Faccilongo Giada, Panza Roberto Maria, Palmieri Andrea | 02'20''10 | | | | |
50m 36.92 (36.92) 100m 1:12.77 (35.85) [1:12.77] 150m 1:46.73 (33.96) 200m 2:20.10 (33.37) [1:07.33] |
| 23 | AICS Nuoto Potenza ASD | Lisanti Francesco, Campochiaro Francesca, Vaccaro Antonella, Pampano Matteo | 02'22''08 | | | | |
50m 31.68 (31.68) 100m 1:10.29 (38.61) [1:10.29] 150m 1:49.11 (38.82) 200m 2:22.08 (32.97) [1:11.79] |
| 24 | Accademia Nuoto Caserta | Romano Amedeo, Esarti Martina, De Angelis Sofia, Cesari Piero | 02'22''50 | | | | |
50m 34.17 (34.17) 100m 1:11.90 (37.73) [1:11.90] 150m 1:47.42 (35.52) 200m 2:22.50 (35.08) [1:10.60] |
| 25 | Energy Group SSD - Montecassino | Morosini Mattia, Angeloni Anastasia, Tamburini Rebecca, Morelli Filippo | 02'23''14 | | | | |
50m 34.74 (34.74) 100m 1:09.86 (35.12) [1:09.86] 150m 1:47.17 (37.31) 200m 2:23.14 (35.97) [1:13.28] |
| 26 | Montecorvino Pugliano M.Sport SRL | Memoli Alessandro, Buscema Corrado, Villecco Rita, Carrafiello Giulia | 02'32''54 | | | | |
50m 37.95 (37.95) 100m 1:18.66 (40.71) [1:18.66] 150m 1:58.01 (39.35) 200m 2:32.54 (34.53) [1:13.88] |
| 27 | AICS Nuoto Potenza ASD | Racioppi Marco, Votta Samanta, Abelardo Martina, Bitetti Elio Nicola | 02'49''60 | | | | |
50m 37.09 (37.09) 100m 1:30.06 (52.97) [1:30.06] 150m 2:15.15 (45.09) 200m 2:49.60 (34.45) [1:19.54] |
