| 1 | CUS Ferrara ASD | Bonizzi Antonia, Prosdocimi Anna, Gruppioni Giulia Elisabetta, Bonora Anita | 05'09''18 | 409 |  |  | |
100m 1:17.53 (1:17.53) [1:17.53] 200m 2:43.93 [1:26.40] 300m 4:03.35 [1:19.42] 400m 5:09.18 [1:05.83] |
| 2 | NS Emilia SSD | Montanari Matilde, Orsi Arianna, Alberghini Giorgia, Vitti Sofia | 05'12''49 | 396 | | | |
100m 1:16.29 (1:16.29) [1:16.29] 200m 2:47.52 [1:31.23] 300m 4:02.84 [1:15.32] 400m 5:12.49 [1:09.65] |
| 3 | Circolo Nuoto UISP Bologna | Ciacciarelli Anastasia, Calzati Melissa, Visigalli Valentina, Balugani Sofia | 05'14''93 | 387 | | | |
100m 1:20.23 (1:20.23) [1:20.23] 200m 2:45.87 [1:25.64] 300m 4:05.48 [1:19.61] 400m 5:14.93 [1:09.45] |
| 4 | Libertas Nuoto Ravenna | Valbonetti Emily, Mazzoni Giulia, Bravi Pondi Rebecca, Battaglia Sara | 05'15''71 | 384 | | | |
100m 1:20.90 (1:20.90) [1:20.90] 200m 2:56.12 [1:35.22] 300m 4:08.38 [1:12.26] 400m 5:15.71 [1:07.33] |
| 5 | Equipe Sportiva SRL SSD | Torri Matilde, Montanini Gaia, Maiorana Viola, Colli Vittoria | 05'15''95 | 383 | | | |
100m 1:18.00 (1:18.00) [1:18.00] 200m 2:49.83 [1:31.83] 300m 4:08.61 [1:18.78] 400m 5:15.95 [1:07.34] |
| 6 | Polisportiva Masi | Shllaku Melissa, Alushaj Alessia, Pau Martina Rita, Bertocchi Liya | 05'22''63 | 360 | | | |
100m 1:22.59 (1:22.59) [1:22.59] 200m 2:53.98 [1:31.39] 300m 4:12.90 [1:18.92] 400m 5:22.63 [1:09.73] |
| 7 | Coopernuoto | Fantuzzi Emily, Lotti Alice, Valentini Rebecca, Pavesi Aurora | 05'24''38 | 354 | | | |
100m 1:20.55 (1:20.55) [1:20.55] 200m 2:52.05 [1:31.50] 300m 4:13.15 [1:21.10] 400m 5:24.38 [1:11.23] |
| 8 | Maranello Nuoto | Balestri Eva, Busi Giada, Savigni Beatrice, Gianni Gloria | 05'29''46 | 338 | | | |
100m 1:20.40 (1:20.40) [1:20.40] 200m 2:55.50 [1:35.10] 300m 4:11.36 [1:15.86] 400m 5:29.46 [1:18.10] |
