| 1 | Forum Sport Center - Roma | Lofrano Alessia, Sangiovanni Alessandro, Gagliardi Matteo, Maggioli Angelica | 01'51''23 | 625 |  |  | |
50m 30.79 (30.79) 100m 1:00.32 (29.53) [1:00.32] 150m 1:24.91 (24.59) 200m 1:51.23 (26.32) [50.91] |
| 2 | In Sport Rane Rosse | Di Giacomantonio Valeria, Polverini Sofia, Petracca Alessio, Mastrorilli Giovanni | 01'51''86 | 615 | | | |
50m 30.22 (30.22) 100m 1:03.17 (32.95) [1:03.17] 150m 1:29.57 (26.40) 200m 1:51.86 (22.29) [48.69] |
| 3 | Olgiata 20.12 SSD | Pasquetti Giuseppe, Braila Kirill, Parla' Sofia, Modanesi Camilla | 01'53''21 | 593 | | | |
50m 26.77 (26.77) 100m 56.30 (29.53) [56.30] 150m 1:25.78 (29.48) 200m 1:53.21 (27.43) [56.91] |
| 4 | The Volume ARL SSD | Lautizi Leonardo, Neccia Martina, Luciani Aurora, Lamonica Miraglio Luca | 01'55''40 | 560 | | | |
50m 27.81 (27.81) 100m 1:01.33 (33.52) [1:01.33] 150m 1:30.66 (29.33) 200m 1:55.40 (24.74) [54.07] |
| 5 | Latina Aquateam ASD | Parlapiano Giulia, Spigariol Matteo, Stipcevich Marco, Becherucci Dora | 01'57''30 | 533 | | | |
50m 30.03 (30.03) 100m 1:01.62 (31.59) [1:01.62] 150m 1:28.51 (26.89) 200m 1:57.30 (28.79) [55.68] |
| 6 | Centro Sport Roma SSD | Arcese Daniele, Glavinas Gabriel, Azzarelli Giulia, Turlea Melissa Elena | 01'57''48 | 531 | | | |
50m 28.49 (28.49) 100m 59.65 (31.16) [59.65] 150m 1:30.28 (30.63) 200m 1:57.48 (27.20) [57.83] |
| 7 | Circolo Canottieri Aniene | Fontana Lucrezia, Munno Alessandro, Poggioli Tiziano, Buzdugan Erica | 01'57''51 | 530 | | | |
50m 32.46 (32.46) 100m 1:02.64 (30.18) [1:02.64] 150m 1:27.59 (24.95) 200m 1:57.51 (29.92) [54.87] |
| 8 | Tortuga Velletri ASD | Abbafati Mariluna, Picca Federico, Bennato Paolo, Castagnaro Alice | 01'58''99 | 511 | | | |
50m 32.77 (32.77) 100m 1:04.59 (31.82) [1:04.59] 150m 1:31.32 (26.73) 200m 1:58.99 (27.67) [54.40] |
| 9 | G. Sport Village SSD - Roma | Fantauzzi Asia, Giovinazzo Gaia, Lobascio Lorenzo, Sava Simone Tomas | 01'59''10 | 509 | | | |
50m 31.56 (31.56) 100m 1:07.55 (35.99) [1:07.55] 150m 1:34.96 (27.41) 200m 1:59.10 (24.14) [51.55] |
| 10 | Polisportiva H2o SSD | Paris Lorenzo, Betti Alessio, Lazzara Caterina, Munno Elena | 01'59''88 | 500 | | | |
50m 29.23 (29.23) 100m 59.92 (30.69) [59.92] 150m 1:31.03 (31.11) 200m 1:59.88 (28.85) [59.96] |
| 11 | Anzio Waterpolis SSD | Torresan Mattia, Sbarrini Morgana Maria, Zappavigna Marco, Trezza Bibiana | 02'01''37 | 481 | | | |
50m 30.21 (30.21) 100m 1:05.78 (35.57) [1:05.78] 150m 1:32.67 (26.89) 200m 2:01.37 (28.70) [55.59] |
| 12 | Eschilo 2 Sport In Progress SSD | Braga Martina, Mecozzi Giacomo Iago, Testaguzza Giorgio, Neroni Ginevra | 02'01''87 | 475 | | | |
50m 31.55 (31.55) 100m 1:03.83 (32.28) [1:03.83] 150m 1:33.12 (29.29) 200m 2:01.87 (28.75) [58.04] |
| 13 | Heaven 2 SSD - Roma | Zuccari Zeno, Altobelli Lorenzo, Patriarca Valentina, Mariani Arianna | 02'02''25 | 471 | | | |
50m 28.97 (28.97) 100m 1:00.90 (31.93) [1:00.90] 150m 1:33.52 (32.62) 200m 2:02.25 (28.73) [1:01.35] |
| 14 | Aurelia Nuoto ASD | Colasanti Claudia, Chiovenda Chiara, Montechiarini Beatrice, Valle Beatrice | 02'06''62 | 424 | | | |
50m 32.39 (32.39) 100m 1:07.68 (35.29) [1:07.68] 150m 1:38.53 (30.85) 200m 2:06.62 (28.09) [58.94] |
