| 1 | Aurelia Nuoto ASD | Caretti Alessia, Sperati Daniele, Aquino Giulia, Chessa Diego | 01'40''13 | 663 |  |  | |
50m 26.56 (26.56) 100m 50.60 (24.04) [50.60] 150m 1:17.48 (26.88) 200m 1:40.13 (22.65) [49.53] |
| 2 | Olgiata 20.12 SSD | Cangialosi Ignazio, Pandolfelli Lara, Corpolongo Giordana, Pasquetti Giuseppe | 01'43''95 | 592 | | | |
50m 25.06 (25.06) 100m 51.64 (26.58) [51.64] 150m 1:18.58 (26.94) 200m 1:43.95 (25.37) [52.31] |
| 3 | Forum Sport Center - Roma | Capozzi Marco, Cristofanilli Simone, Maggioli Angelica, Canciamilla Claudia | 01'44''49 | 583 | | | |
50m 24.92 (24.92) 100m 48.79 (23.87) [48.79] 150m 1:16.80 (28.01) 200m 1:44.49 (27.69) [55.70] |
| | Primavera Campus SSD SRL | Bagaglini Giulia, Cornacchione Margherita, Bazzoni Federico, Spano Emanuele | 01'44''49 | 583 | | | |
50m 28.05 (28.05) 100m 56.34 (28.29) [56.34] 150m 1:20.46 (24.12) 200m 1:44.49 (24.03) [48.15] |
| 5 | Centro Nuoto Anagni ASD | D'Agostini Matteo, Galuppi Christian, Colasanti Chiara, Martini Viola | 01'45''76 | 563 | | | |
50m 25.05 (25.05) 100m 49.70 (24.65) [49.70] 150m 1:17.20 (27.50) 200m 1:45.76 (28.56) [56.06] |
| 6 | Primavera Campus SSD SRL | Amato Ludovica, Bagaglini Ginevra, Clazzer Valerio, Cirelli Marco | 01'45''82 | 562 | | | |
50m 27.98 (27.98) 100m 56.36 (28.38) [56.36] 150m 1:20.90 (24.54) 200m 1:45.82 (24.92) [49.46] |
| 7 | The Volume ARL SSD | Lamonica Miraglio Luca, Neccia Martina, Cedroni Michela, Lautizi Leonardo | 01'45''93 | 560 | | | |
50m 25.18 (25.18) 100m 52.65 (27.47) [52.65] 150m 1:21.10 (28.45) 200m 1:45.93 (24.83) [53.28] |
| 8 | Ippocampo SSD - Roma | Rossi Giacomo, Vita Diletta, Calderoni Alessandro, Parri Martina | 01'47''05 | 542 | | | |
50m 25.15 (25.15) 100m 54.31 (29.16) [54.31] 150m 1:19.05 (24.74) 200m 1:47.05 (28.00) [52.74] |
| 9 | Physical Village Roma SSD | Torri Flavio, Cinti Gabriele, Dimonopoli Emma, Di Stefano Flaminia | 01'49''22 | 511 | | | |
50m 25.81 (25.81) 100m 51.62 (25.81) [51.62] 150m 1:20.84 (29.22) 200m 1:49.22 (28.38) [57.60] |
| 10 | SIS Roma SSD | Materazzo Jacopo, Madaghiele Angelica, Vannozzi Nicole, Ippoliti Mattia | 01'49''76 | 503 | | | |
50m 26.48 (26.48) 100m 54.83 (28.35) [54.83] 150m 1:23.41 (28.58) 200m 1:49.76 (26.35) [54.93] |
| 11 | Nuova Partenope Nuoto SSD | Maddaluno Anna Emma, Abbruzzese Giulia, Montanaro Giuseppe, Paganini Alessandro | 01'54''62 | 442 | | | |
50m 30.11 (30.11) 100m 1:00.34 (30.23) [1:00.34] 150m 1:26.63 (26.29) 200m 1:54.62 (27.99) [54.28] |
| 12 | Quattro Star SSD | Anghel Serena, Testa Alessandro, Pagani Eleonora, Volpi Alessio | 01'55''83 | 428 | | | |
50m 30.76 (30.76) 100m 58.41 (27.65) [58.41] 150m 1:29.62 (31.21) 200m 1:55.83 (26.21) [57.42] |
| 13 | Physical Village Roma SSD | Bernardini Simone, Pasqua Simone, Mozzoni Sara, Onofri Marika | 01'57''68 | 408 | | | |
50m 28.38 (28.38) 100m 58.07 (29.69) [58.07] 150m 1:27.99 (29.92) 200m 1:57.68 (29.69) [59.61] |
| 14 | Gladiators Swim God SSD | Ramadu' Emanuele, Di Pietro Simone Maria, Greco Emma, Neri Anna | 01'58''05 | 404 | | | |
50m 28.50 (28.50) 100m 55.77 (27.27) [55.77] 150m 1:26.57 (30.80) 200m 1:58.05 (31.48) [1:02.28] |
